Abstract
Serum of multiparous women can contain HLA polyclonal antibodies which can be used as reagents for serological typing of HLA class I and class II. The aim of this study was to investigate an alloimmunization of multiparous women and to identify HLA polyclonal antibodies which could be useful as laboratory reagents in view of the future HLA unit of the Department of Immunology at Abidjan. In an experimental and analytic study, we screened HLA antibodies using microlymphocytotoxicity test on sera of 121 multiparous women aged from 18 to 70 years old, in apparent good health, transfused or no-transfused and with at least two parities. 37.19% of the multiparous women were alloimmunized. 13 women were sensitized to HLA class I, 18 to HLA class II and 14 were both sensitized to class I and class II. Specificity of the screened antibodies was identified at 17.78%. HLA-B7 specificity (Class I) was 37.5% and class II specificity (HLA-DR4, DR7, DR13, DR13+14, DRw52) was 62.5%. Specificity was evaluated by the correlation coefficient. For class I specificity correlation coefficients were ranged from 0.79 to 1, while for class II specificities, correlation coefficients were ranged from 0.54 to 0.9. Screening of HLA antibodies on multiparous women at Abidjan was positive and specificities were determined. This enabled authors to the possibility of using multiparous women sera as a source of production of polyclonal antibodies for serological HLA typing in the HLA unit of the Department of Immunology at Abidjan.
Highlights
The human leukocyte antigen (HLA) system represents a family of polymorphic cell surface molecules involved in the presentation of antigen to T cells and play a central role in the induction and regulation of immune responses [1, 2]
A first step was to screen anti-HLA class I and class II by microlymphocytotoxicity method based on the lysis of lymphocytes panel by specific HLA antibodies contained in the serum in presence of rabbit complement
Paternal HLA molecules expressed by fetal cells that pass through the maternal bloodstream induce blast transformation of no histocompatible CD4+ which produce among other cytokines, IL-4 and IL-5 that stimulate production of antibodies by B lymphocytes and plasma cell progeny [11]
Summary
The human leukocyte antigen (HLA) system represents a family of polymorphic cell surface molecules involved in the presentation of antigen to T cells and play a central role in the induction and regulation of immune responses [1, 2]. Anti-HLA antibodies can be identified in approximately 20% of pregnant women [4]. Multiparous women can be a source of producing HLA antibodies. The production, typing and identification of HLA antibodies are part of the routine laboratory tests performed for blood donors in developed countries [5]. Technological advances in the health sector of our country and organ transplant prospects dictate that we can routinely perform the serological typing of HLA antigens, the need of antiHLA specific antibody. Serum of multiparous women may contain anti-HLA polyclonal antibodies, which can be used as reagents for serological typing of HLA class I and class II [4]. HLA antibodies detected both in serum of multiparous women, polyclonal antibodies, are excellent reagents for HLA typing with a reasonable cost.
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