Abstract
BackgroundHantaan virus (HTNV) infection in humans is a serious public health concern in Asia. A potent T cell activation peptide vaccine from HTNV structure protein represents a promising immunotherapy for disease control. However, the T cell epitopes of the HTNV restricted by the HLA alleles and the role of epitope-specific T cell response after HTNV infection remain largely unexplored.Methodology/Principal FindingsFive well-conserved novel CD8+ T-cell epitopes of the HTNV nucleoprotein restricted by the most popular HLA alleles in Chinese Han population were defined with interferon-γ enzyme-linked immunospot assay in 37 patients infected with HTNV during hospitalization. Two epitopes aa129–aa137 and aa131–aa139 restricted by HLA-A2 and B35, respectively, were selected to evaluate the epitope-specific CD8+ T-cell response. HLA-peptide pentamer complex staining showed that the frequency of single epitope-specific CD8+ T cell could be detected in patients (95% confidence interval for aa129–aa137: 0.080%–0.208%; for aa131–aa139: 0.030%–0.094%). The frequency of epitope-specific pentamer+ CD8+ T-cell response was much higher in mild/moderate patients than in severe/critical ones at the acute stage of the disease. Moreover, the frequency of epitope-specific CD8+ T cells at acute stage was inversely associated with the peak level of serum creatinine and was positively associated with the nadir platelet counts during the hospitalization. The intracellular cytokine staining and the proliferation assay showed that the effective epitope-specific CD8+ T cells were characterized with the production of interferon-γ, expression of CD69 and the strong capacity of proliferation.Conclusion/SignificanceThe novel HLA class I restricted HTNV nucleoprotein epitopes-specific CD8+ T-cell responses would be closely related with the progression and the severity of the disease, which could provide the first step toward effective peptide vaccine development against HTNV infection in humans.
Highlights
Hantaan virus (HTNV), the prototype member of the genus Hantavirus of the family Bunyaviridae, was first isolated in Korea in 1978 and can cause a severe disease of hemorrhagic fever with renal syndrome (HFRS) in humans [1]
Pathogenic Hantaviruses have emerged as an increasing threat to human health, as are they the causative agents of HFRS in Asia and Europe that are associated with HTNV, Seoul virus, Dobrava virus, and Puumala virus (PUUV) infections [4,5,6], and they can induce the severe hantavirus pulmonary syndrome (HPS) in North and South America, which is caused by Sin Nombre virus (SNV) and Andes virus serotypes [7,8]
Since effective prevention is not available currently and the non-specific symptoms at the early stage of the disease always lead to the delay of visiting to hospital or misdiagnosis, alternative vaccinations against HTNV are of priority to overcome the problem
Summary
Hantaan virus (HTNV), the prototype member of the genus Hantavirus of the family Bunyaviridae, was first isolated in Korea in 1978 and can cause a severe disease of hemorrhagic fever with renal syndrome (HFRS) in humans [1]. Pathogenic Hantaviruses have emerged as an increasing threat to human health, as are they the causative agents of HFRS in Asia and Europe that are associated with HTNV, Seoul virus, Dobrava virus, and Puumala virus (PUUV) infections [4,5,6], and they can induce the severe hantavirus pulmonary syndrome (HPS) in North and South America, which is caused by Sin Nombre virus (SNV) and Andes virus serotypes [7,8]. The T cell epitopes of the HTNV restricted by the HLA alleles and the role of epitope-specific T cell response after HTNV infection remain largely unexplored
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