Abstract
Objective To detect and analyze vpr gene diversity of human immunodeficiency virus type 1(HIV-1)from patients with HIV in China.Different of mutated sites and molecular epidemic charac-tefisfics between vpr gene in China and abroad were compared to explore pathogenesis mechanisms of HIV-1 and possible gene therapy sites in the future.Methods Reverse transcription polymerase chain reaction uals.BLAST,Clustal W and Mega biological analysis software were applied to analyze sequences,and identify the characteristics of HIV-1 vpr gene polymorphism and its clinical significance in China.The uproo-ted tree was calculated according to the neighbor-joining method.Results HIV-1 vpr subtypes in China were CRFO1-AE(51.19%),subtypes C(21.43%),subtype B(18.18%),CRFO3-AB(2.60%)and CRFO8-BC(2.60%).Nine sites in vpr deduced amino acid sequences showed more than 20%mutated ra-tio.Conclusion This paper firsdy analyzed HIV-1 vpr gene diversity and the characteristics of vpr gene tree from Chinese patients.Some sites in vpr deduced amino acid sequences showed obvious mutated ratio,which were possibly correlated with clinical manifestation of HIV-1 infected individuals. Key words: vpr Gene Products,human Immunedeficiency Virus/GE; HIV Infections/GE; Poly-morphism,genetic
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