Abstract

Human immunodeficiency virus (HIV) is the chief contributor to global burden of disease. In 2010, HIV was the fifth leading cause of disability-adjusted life years in people of all ages and leading cause for people aged 30–44 years. It is classified as a member of the family Retroviridae and genus Lentivirus based on the biological, morphological, and genetic properties. It infects different cells of the immune system, such as CD4+ T cells (T-helper cells), dendritic cells, and macrophages. HIV has two subtypes: HIV-1 and HIV-2. Among these strains, HIV-1 is the most virulent and pathogenic. Advanced diagnostic methods are exploring new ways of treatment and contributing in the reduction of HIV cases. The diagnostic techniques like PCR, rapid test, EIA, p24 antigen, and western blot have markedly upgraded the diagnosis of HIV. Antiretroviral therapy and vaccines are promising candidates in providing therapeutic and preventive regimes, respectively. Invention of CRISPR/Cas9 is a breakthrough in the field of HIV disease management.

Highlights

  • Hiv Diagnosis and Treatment through Advanced TechnologiesHafiza Fizzah Zulfiqar, Aneeqa Javed, Sumbal, Bakht Afroze, Qurban Ali1*, Khadija Akbar, Tariq Nadeem, Muhammad Adeel Rana, Zaheer Ahmad Nazar, Idrees Ahmad Nasir and Tayyab Husnain

  • Human immunodeficiency virus (HIV) originates from a monkey infecting virus, simian immunodeficiency virus (SIV), and a number of theories have been described in this regard

  • This treatment therapy was known as highly active antiretroviral therapy (HAART), and it has the potential to reduce the mortality and morbidity related to HIV-1 infection [100]

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Summary

Hiv Diagnosis and Treatment through Advanced Technologies

Hafiza Fizzah Zulfiqar, Aneeqa Javed, Sumbal, Bakht Afroze, Qurban Ali1*, Khadija Akbar, Tariq Nadeem, Muhammad Adeel Rana, Zaheer Ahmad Nazar, Idrees Ahmad Nasir and Tayyab Husnain. In 2010, HIV was the fifth leading cause of disability-adjusted life years in people of all ages and leading cause for people aged 30–44 years. It is classified as a member of the family Retroviridae and genus Lentivirus based on the biological, morphological, and genetic properties. It infects different cells of the immune system, such as CD4+ T cells (T-helper cells), dendritic cells, and macrophages. HIV has two subtypes: HIV-1 and HIV2. Among these strains, HIV-1 is the most virulent and pathogenic.

BACKGROUND
HIV TROPISM
HIV STRUCTURE AND GENOME ORGANIZATION
HIV LIFE CYCLE
IN HIV SUSCEPTIBILITY AND DISEASE
SNP ASSOCIATED WITH HIV
SNP location
Type of test
Rapid Tests
Diagnostic Window
EIA for HIV
Western Blot
Qualitative PCR
Quantitative RNA PCR and Genotyping
Induction of Neutralizing Antibodies
Recombinant Viral Vectors
Types of Antiretroviral Agents
Integrase Inhibitors
Protease Inhibitors
GENE EDITING TOOLS FOR HIV
Zinc Finger Nucleases
Findings
CONCLUSION
Full Text
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