Abstract
Materials and methods The process through which HIV-1 is endocytosed was studied using different reagents (e.g. chlorpromazine, cholera toxin B, water-soluble cholesterol, colchicine, cytochalasin B, filipin, jasplakinolide, methyl-beta-cyclodextrin, paclitaxel, and vinblastine) and experimental strategies (e.g. transfection of JAR cells with various expression vectors, virus internalization test, infection assay, confocal laser scanning, co-localization analysis and digital image preparation).
Highlights
In human trophoblastic cells, a correlation between early endosomal trafficking of HIV-1 and virus infection was previously documented
If HIV-1 is massively internalized in these cells, the endocytic pathway(s) responsible for viral uptake is still undefined
Even though HIV-1 did not initially co-localize with transferrin, some virions migrate at later time points to transferrin-enriched endosomes, suggesting an unusual transit from the non-classical pathway to early endosomes
Summary
Address: Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval, and Département de Biologie médicale, Université Laval, Québec (QC), Canada. Published: 9 April 2008 Retrovirology 2008, 5(Suppl 1):O5 doi:10.1186/1742-4690-5-S1-O5. Maternal chronic viral infections transmitted to infants: from mechanisms to prevention and care Meeting abstracts - A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1742-4690-5-S1-info.pdf
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