Abstract
The HIV-1 Gag protein is responsible for genomic RNA (gRNA) packaging and immature viral particle assembly. Although the presence of gRNA in virions is required for viral infectivity, in its absence, Gag can assemble around cellular RNAs and form particles resembling gRNA-containing particles. When gRNA is expressed, it is selectively packaged despite the presence of excess host RNA, but how it is selectively packaged is not understood. Specific recognition of a gRNA packaging signal (Psi) has been proposed to stimulate the efficient nucleation of viral assembly. However, the heterogeneity of Gag-RNA interactions renders capturing this transient nucleation complex using traditional structural biology approaches challenging. Here, we used native MS to investigate RNA binding of wild-type (WT) Gag and Gag lacking the p6 domain (GagΔp6). Both proteins bind to Psi RNA primarily as dimers, but to a control RNA primarily as monomers. The dimeric complexes on Psi RNA require an intact dimer interface within Gag. GagΔp6 binds to Psi RNA with high specificity in vitro and also selectively packages gRNA in particles produced in mammalian cells. These studies provide direct support for the idea that Gag binding to Psi specifically promotes nucleation of Gag-Gag interactions at the early stages of immature viral particle assembly in a p6-independent manner.
Highlights
The ability to select the viral genomic RNA for packaging into the assembling virus particle is absolutely necessary for specific replication of HIV-1 and other retroviruses
Because Gag can assemble around non-genomic RNA (gRNA) in the cell, we hypothesize that Gag–Gag interactions are facilitated by NC domain binding to packaging signal” (Psi) in a manner that is distinct from binding to non-Psi RNA sequences
We examined the oligomeric state of free Gag proteins at multiple concentrations in the absence of any nucleic acids
Summary
The ability to select the viral genomic RNA (gRNA) for packaging into the assembling virus particle is absolutely necessary for specific replication of HIV-1 and other retroviruses. Because Gag can assemble around non-gRNA in the cell, we hypothesize that Gag–Gag interactions are facilitated by NC domain binding to Psi in a manner that is distinct from binding to non-Psi RNA sequences. We analyzed the oligomeric state of Gag in the presence of Psi RNA and a control RNA, TARpolyA (Fig. 1B).
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