Abstract
Background During HIV-1 infection, dendritic cells (DC) facilitate dissemination of HIV-1 while trying to trigger adaptive antiviral immune responses. We examined whether increased HIV-1 capture in DC matured with lipopolysaccharide (LPS) results in more efficient antigen presentation to HIV-1–specific CD4 + and CD8 + T cells. In order to block the DC-mediated trans-infection of HIV-1 and maximize antigen loading, we also evaluated a non-infectious integrase-deficient HIV-1 isolate, the HIVNL4-3ΔIN. Methods Immature DC (iDC), mature DC (mDC) activated with IL-1b, TNF-a, IL-6, and PGE2 (ITIP) or LPS during viral uptake, and fully mDC matured with ITIP or with LPS for 48 h before viral loading were tested. Antigen presentation to HIV-1-specific CD4 + and CD8 + T cell clones was quantified by IFN-g ELISPOT. DC-associated p24 Gag HIV-1 and DC-mediated HIV-1 trans-infection were also evaluated in parallel. Results
Highlights
During HIV-1 infection, dendritic cells (DC) facilitate dissemination of HIV-1 while trying to trigger adaptive antiviral immune responses
We examined whether increased HIV-1 capture in DC matured with lipopolysaccharide (LPS) results in more efficient antigen presentation to HIV-1–specific CD4+ and CD8+ T cells
We showed that higher viral capture of DC did not guarantee better antigen presentation or T-cell activation
Summary
HIV-1 capture and antigen presentation by dendritic cells: enhanced viral capture does not correlate with better T-Cell activation. M Rodriguez-Plata1*, A Urrutia, S Cardinaud, M Buzon, N Izquierdo-Useros, JG Prado, M Puertas, I Erkizia, P Coulon, S Cedeño, B Clotet, A Moris, J Martinez-Picado
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