Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis

Abstract

Promoter-driven GUS (beta-glucuronidase) activity is the most commonly used technique for tissue-specific expression patterns in Arabidopsis. In this procedure, GUS enzyme converts 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) to a blue product. The staining is very sensitive. Processed samples can be examined under dissecting microscope or Differential Interference Contrast (Nomaski) microscope for bright blue color over cleared transparent background. Note this assay does not provide accurate information to subcellular levels., 启动子驱动的GUS（β-葡糖醛酸糖苷酶）活性是拟南芥中组织特异性表达模式最常用的技术。 在该程序中，GUS酶将5-溴-4-氯-3-吲哚基葡糖苷酸（X-Gluc）转化为蓝色产物。 染色非常敏感。 处理的样品可以在解剖显微镜或微分干涉对比（Nomaski）显微镜下在透明背景上检查明亮的蓝色。 注意这个测定不提供准确的信息亚细胞水平。