Abstract
In conclusion, the use of glycerol or dimethyl sulfoxide has permitted successful freezing, storage, and thawing of most, but not all, animal cell cultures. Storage in liquid nitrogen without detectable change in observable characteristics appears to be indefinite. Methods are empiric, understanding of the basic physics and chemistry involved are fragmentary, and results cannot always be exactly duplicated within one laboratory or in different laboratories. These problems will be solved in the future by the combined application of many disciplines with overlapping interests in molecular biology. In the meantime, present methods permit indefinite cold storage of most cell cultures with a great saving in labor and inconvenience, while avoiding accidental loss, contamination, or mutation of the cells during repeated serial passage.
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