HISTOPATOLOGY, BLOOD UREA NITROGEN AND CREATININE PROFILE OF RATS WITH UNILATERAL URETERAL LIGATION

Abstract

Abstract Ureteral obstruction is an urinary tract disorder that can occur in animals and human. The effect of total unilateral ureteral obstruction on the kidneys is fatal if not immediately treated because it can cause ipsilateral or contralateral kidney damage. This study was conducted to determine histopathological features, and blood urea nitrogen (BUN) and creatinine levels in white rats that had unilateral ureteral obstruction. Thirty-six 2,5 months female Sprague Dawley rats were divided into 3 groups. Group I (control) was operated laparotomy. Group II was operated by ligating the right ureter in the proximal part. Group III was operated by ligating the right ureter in the distal part. One week after ligation three rats were taken randomly from each group and their blood was taken for BUN and creatinine analysis. The right and left kidneys were taken to make histopathological preparations. The same treatment was carried out at 2 nd , 3 rd , and 4 th weeks after surgery. Statistical analysis showed an increase in BUN and creatinine. Ipsilateral renal hydronephrosis occurs from the first week after unilateral ureteral ligation, which is characterized by renal pelvic dilatation, parenchymal depletion, vacuolization and fluid deposits in the right kidney. The ipsilateral renal histopathology examination showed glomerular atrophy and tubular dilatation in the first week after ligation. Cysts formation and interstitial fibrosis occurred on the 2 nd to 4 th week of the ipsilateral kidney and geting worse along with the length of obstruction time. Compensatory reaction occurred in the contralateral kidney was found with dilatation of tubulus distal and proximal from the first week after ligation. This study showed that unilateral ureteral ligation in the proximal and distal regions causes an increase in BUN and creatinine and hydronephrosis of the ipsilateral kidney which appears macroscopically and histopathologically. Keywords: ureteral obstruction, kidney, blood urea nitrogen, creatinine, histopathology