Histopatological Impact of Malathion on the Reproductive Organs of Freshwater Crabs Barytelhusa Cunicularis (Westwood, 1836)

Abstract

Crabs are particularly useful in aquatic environment and play a very important role in the ecosystem processes and good indicator for polluted condition. The terrestrial crab plays a significant modal in the study of population and also beneficial for human activities. The present study deals with the histochemical changes of organophosphate, malathion in the freshwater crab Barytelphusa cunicularis. Live specimens of Barytelphusa cunicularis were collected from local wet areas of Marodasector, Bhilai, (C.G). The crabs were sexed on the basis of the shape of the abdomen. Average weight of crabs varies from (45-50 gms), the carapace length and carapace width varies from 3.20-4.50mm and 4.25-5.80mm respectively. The crabs were acclimatized in the laboratory condition, and kept in two groups the control group set free from Malathion and the experimental group was exposed to malathion for LC50 at different concentration 0.45ppm, 0.30ppm, 0.26ppm and 0.25ppm for 24hrs, 48hrs, 72hrs and 96hrs respectively. The sections of 5µm were taken for the histopathological examination of testis and ovary; affected due to the impact of Malathion, the testicular follicular cells interstitials cells and nutritive cells were decreased and distracted number of sperm cells it exhibited irregular arrangement of nucleus, in the ovary epithelial layer destruction, degeneration of oocytes, Shrinkage in ooplasm, vacuolization and reduction in cytoplasmic material. The result suggests that rapid utilization to meet the energy demands under the impact of malathion.