Histopathological view of silver barb (Barbonymus gonionotus) kidney that contaminated by heavy metal Cadmium (Cd) in Tempe Lake, Wajo Distric

Abstract

Start Silver barb (Barbonymus gonionotus) is widely consumed by the community and is the dominant type of fish caught throughout the year in Lake Tempe. The purpose of this study was to determine the histopathological view of the kidneys in silver barb contaminated with heavy metal cadmium (Cd). Samples were used is 15 silver barb with each of 5 kidney samples at each station. Measurement of heavy metal content was carried out with an Atomic Absorption Spectrophotometer (AAS) and obtained cadmium (Cd) concentrations in the kidney organs of contaminated fish which were 0.29 - 1.94 µg / g with an average of 0.77 ± 0.12 µg / g. Kidney organ preparations were fixed using 10% neutral buffered formalin (NBF), dehydration using graded alcohol, embedding using paraffin, cutting with a thickness of 5 μm and stained with haematoxilin eosin and then observed. Analysis of the data used is descriptive qualitative. Based on observations obtained by damage or histopathology that occurs in the kidneys namely fat degeneration, connective tissue formation, necrosis, hemorrhage and an increase in the number of melanomacrophages. The level of damage to the tissue depends on the concentration of the metal contaminated in the fish’s organs. Damages that occur are thought to be caused by exposure to heavy metals dissolved in the waters of the fish ecosystem which have passed the threshold.