Histopathological study of splenitis in cattle induced by traumatic foreign body penetration

Abstract

Aim: A detailed post mortem examination was conducted on the animal to know the cause of the death. Materials and Methods: Detailed post-mortem examination was carried out. The Impression smears from the affected organs was taken and subjected to Gram's staining technique for preliminary identification of the micro-organism. The suspected tissues were processed for histological investigation by formalin fixation and paraffin embedding technique as explained by Luna. Results: Hardware diseases in cattle are a common feature in developing countries where organized farming is still not an adopted practice of management. During post mortem examination, a case of foreign body penetration induced splenitis was encountered. A large irregular abscess cavity was found with greenish liquefactive material found wherever the foreign body had penetrated. Multiple focal abscesses were found in the parenchyma along with congestion and edema. The histopathological investigation revealed inflammation with neutrophilic liquefactive debris, mild fibrosis, vascular sclerosis, focal lymphocytolysis and numerous chains of regular sized bacilli in the parenchyma, determined to be Bacillus spp. Trauma due to foreign body is rare in spleen due to its anatomical location. Conclusion: Foreign body penetration induced splenitis was encountered. The histopathological investigation revealed inflammation with neutrophilic liquefactive debris, mild fibrosis, vascular sclerosis, focal lymphocytolysis and numerous chains of regular sized bacilli in the parenchyma, determined to be Bacillus spp.