Abstract

ABSTRACTThe aim of the present study was to assess the systemic effect of thymoquinone (TQ) on bone healing by starting TQ administration, either 40 days before, or on the day of the surgical procedure and continuing during the healing period of 28 days. Eighteen experimental rats were divided into three groups and defects were created in their tibias. The following procedures were performed for each group: Control group (C): No systemic drug administration (n = 6); Test group 1 (T1): Systemic TQ was administered daily starting 40 days before creation of the defect and additionally during the post-operative healing period of 28 days (n = 6); Test group 2 (T2): Systemic TQ was administered daily after creation of the defect and during the healing period of 28 days (n = 6). Quantitative measurement for new bone formation, osteoblast lining and semi-quantitative measurement of capillary intensities were examined and statistically analysed. There was a significant increase in the ratio of new bone per total defect area and new bone trabeculae lined by active osteoblasts in both test groups (T1 and T2) compared to control group (p < 0.05). However the difference between T1 and T2 was not statistically significant. TQ-administered groups also showed an increase in capillary intensity in the defect area compared to the control group (p < 0.05). Systemic administration of TQ either starting 40 days before or on the day of surgery accelerated new bone formation in a rat model and can be advocated as an adjunct to expedite bone healing.

Highlights

  • Rehabilitation of the bone defects is a common procedure in oral surgery

  • The present study investigated the systemic effect of TQ on bone healing in surgically created critical size rat tibia defects

  • The tibial proximal epiphysis has a medial face that is suitable for inducing bone defects, as it has a wide and slightly convex surface that is devoid of muscle insertion [27]

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Summary

Introduction

Rehabilitation of the bone defects is a common procedure in oral surgery. There have been an increasing number of studies on this subject [1]. Several approaches, such as the local or systemic administration of growth factors [2,3] or mesenchymal stem cells [4,5,6], can represent advantageous alternatives to bone graft. The use of natural products as an alternative to conventional treatment in healing and treatment on various diseases, as well as bone defects have been on the rise in the last few decades [7,8]. The exact diameter of critical and non-critical size defects is still being debated [10]

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