Abstract
To assess the long-term effect on the tissues of marine fish, Atlantic cod, Gadus morhua L., were exposed to water-soluble fractions of Venezuelan and Hibernia crude oils at concentrations of 50–300 ppb for 12–13 weeks in a flow-through seawater system. Histopathological changes in oil-exposed fish included increased numbers of mucus-producing epithelial cells, capillary dilation, lamellar hyperplasia, and fusion of adjacent filaments in gills, microvesicular formation in hepatocytes, delayed spermatogenesis with intratubular multinucleated giant cells, and an increase of melanomacrophage centers in the spleen and kidney. Lesions were more prevalent and severe in fish exposed to Hibernia crude than to Venezuelan crude at a similar concentration.
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