Histopathological changes in submandibular gland and dorsal tongue of experimental rats due to prolonged tramadol intake focusing on novel modulatory effect of 10-dehydrogingerdione

Abstract

ObjectivesThis project aims to develop a framework to illustrate the degenerative effects induced by prolonged tramadol intake in salivary glands and tongue tissues. We strive in this work to investigate the probable role of 10-dehydrogingerdione (10-DHGD) in regeneration of these tissues. DesignForty male albino rats were designated for the study and categorized into four groups. Group (1) received no drugs and served as normal control group. Group (2) received tramadol intra peritoneal (20 mg /kg) body weight daily for 45 days. Group (3) received freshly prepared 10-DHGD orally in a dose level (10 mg /kg). Group (4) received combination of tramadol and 10 DHGD for 45 days. Histological examination is that routine testing that was done in all studied subjects to demonstrate any cytological changes with hematoxylin and eosin (H&E) in the submandibular glands and dorsal tongue tissues along with histochemical investigation using periodic acid-Schiff (PAS) and immunohistochemical presentation of Caspase-3. ResultsSubmandibular salivary glands and dorsal tongue tissues showed degenerative changes in tramadol treated group while control and 10-DHGD groups presented with no cytological or morphological changes. Histochemical investigation revealed marked reduction in PAS staining reaction in tramadol group as compared to other studied groups. Regarding to immunoreactivity of caspase-3 when all groups were compared, the differences in mean values of area percentage were statistically significant. ConclusionsTramadol provoked oxidative damage and apoptosis in oral tissues, which significantly decreased by 10-DHGD intake as it may exert an ameliorative effect that help alleviating these degenerative effects.