Histopathologic study on the effects of methionine in reduction of hepatotoxicity and gastritis due to co-administration of gentamicin and indomethacin in rats

Abstract

Gentamicin is a wide spectrum aminoglycoside used against gram negative bacterial infections, therapeutic does of this drug resulted in nephrotoxicity and hepatotoxicity. Indomethacin is one of the NSAIDs with side effects on stomach, liver and Kidney. Co-administration of indomethacin and gentamicin may resulted in exaggeration of their side efects .In this research, 42 male Sprague dawley rats were divided randomly into 6 equal groups. In the period of 14 days group 1-6 received normal saline (I.P), gentamicin (I.M), indomethaci (I.M), gentamicin (I.M) plus indomethacin (I.M), L-methionine (I.P), and gentamicin (I.M) plus indomethacin (I.M) and L-methionine(I.P) respectively once a day. In day 15, rats were euthanized and histopathologic slides of the liver and stomach were prepared. Histopathologic examination revealed and edema in tunica mucosa and submucosa of stomach in group 3 and 4, and mild hemorrahage in tunica mucosa of stomach of group 3 .In group 2 and 4 capillarization , deformed hepatocytes , apoptosis and mononuclear cell infiltration in portal area were seen in liver . Fibrosis of portal area was obvious in group 4. For the first time the results of the peresent study showed that methionine can prevent form side effects of Co-administration of indomethacin and gentamicin in liver and stomach.