Abstract
Fibrosarcoma is characterized as an invasive nodular tumor of mesodermal origin, with elongated normochromatic nuclei. The histopathologic analysis using specific staining techniques is efficient in detailing the neoplastic architecture and the relations with surrounding tissues. In the present study, the canine skin fibrosarcoma was histologically characterized using Hematoxilin-Eosin stain, Mallory’s Trichrome stain and Shorr’s Trichrome stain as routine staining for conjunctive tissue, Toluidine Blue to highlight the mitosis and Picrosirius-Hematoxilin Trichrome to characterize the collagen type. It was concluded that the Picrosirius stain characterized the existing collagen type as type I and III; the Toluidine Blue staining highlighted the mitoses together with Shorr’s staining, as this one also provided a marking for the conjunctive tissue and the formed bands. The Mallory’s staining technique highlighted the conjunctive tissue and the bands that are spread; the Hematoxilin-Eosin stain highlighted with emphasis the neoplastic cells.
Highlights
There has been some time since a more interest in veterinary oncology started, especially in pets, in which a higher occurrence of the disease (MULLER; DALMOLIN, 1970; MOULTON, 1990).The neoplasm can be defined as an abnormal growth of the cells or a secondary growth of the autonomous tissue of progressive character
The literature that deals with this subject in domestic animals is relatively expressive and it is where it can be observed that the skin is the main site of neoplasias, especially in dogs (MOULTON, 1990)
This study aims at characterizing histologically the skin fibrosarcoma in dogs (Canis familiares, Linnaeus, 1758), using staining techniques specific for conjunctive tissue and characterizing the type of collagen from the fibrosarcoma through Picrosirius-Hematoxilin Trichrome
Summary
There has been some time since a more interest in veterinary oncology started, especially in pets, in which a higher occurrence of the disease (MULLER; DALMOLIN, 1970; MOULTON, 1990).The neoplasm can be defined as an abnormal growth of the cells or a secondary growth of the autonomous tissue of progressive character. It appears spontaneously from the affected animal’s cells and it is different from normal tissues for its morphological and functional characters. According Magalhães et al (2001), the fibrosarcoma, as well as the other tumors, can be Histopathologic study of cutaneous fibrosarcoma in dogs (Canis familiares, Linnaeus, 1758)
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