Abstract
To evaluate histopathologic alterations of the peritoneum exposed to heat shock. Sixty rats were randomly distributed into 6 groups: Heat Shock (HS), High Temperature (HT), Body Temperature (BT), Temperature 0oC (TZ), Sham (SH) and Control (CG) with 10 animals each. The peritoneal cavity of animals from groups HS, HT, BT and TZ was irrigated with NaCl solution 0.9% at temperatures 50 degrees C, 0 degrees C, 50 degrees C, 37 degrees C and 0 degrees C, respectively. For animals from group SH, the procedures were simulated and those from group CG, laparotomy and biopsies were conducted. Twenty-four hours later, biopsies of the peritoneum for exams under light and electronic microscopy were performed. Edema was found in groups HS 80%, HT 60%, BT 30% TZ 70%, SH 40% and CG 30%. Vascular congestion was found in groups HS 20%, HT 30%, BT 10% and TZ 20%. Erythrocyte extravasation was found in groups HT 60% and SH 10%. Mesothelium destruction was found in 100% of specimens from groups HS, HT, BT, TZ, SH and CG 90%. Necrosis was found in groups HS 30%, HT 20% and BT 10%. The mean peritoneal thickness ranged from 42.26 microm (TZ) to 26.42 microm (CG). The heat shock caused no deaths, but promoted significant peritoneal edema without affecting the other histopathologic indicatives.
Highlights
Heat shock may be stimulated in the peritoneal cavity (PC) through irrigation with NaCl solution 0.9% at different temperatures employed in peritoneal lavage (PL)
The employment of saline solution for PL at high temperature followed by lavage with solutions at low temperature, the heat shock, could produce damages to microorganisms found in the peritoneum, to the milk pasteurization process[22], but it could cause histopathologic alterations in the peritoneum, muscles or in the intestine wall as those observed in response to the exposition of these tissues to biological agents[9]
When these histopathologic indicatives found for the other groups High Temperature (HT), Body Temperature (BT) and TZ were compared to those observed for groups SH and CG, no significant differences were found, except for the erythrocyte extravasation incidence for group HT, which was significant in relation to SH and CG, justifying the necessity to control the temperature of saline solutions employed, as proposed by Silva et al.[19]
Summary
Heat shock may be stimulated in the peritoneal cavity (PC) through irrigation with NaCl solution 0.9% (saline) at different temperatures employed in peritoneal lavage (PL). The employment of saline solution for PL at high temperature followed by lavage with solutions at low temperature, the heat shock, could produce damages to microorganisms found in the peritoneum, to the milk pasteurization process[22], but it could cause histopathologic alterations in the peritoneum, muscles or in the intestine wall as those observed in response to the exposition of these tissues to biological agents[9] In this context, in an experimental research, Silva, et al.[19] reported that when the saline solution at temperature of 60oC used for PL in rats remained within the PC for 1 min could cause histopathologic alterations in the peritoneum and in the muscular fibers of the PC posterior wall perceived at light microscopy with mortality rate of 66% at the first 24 hours. The objective of the present study was to evaluate possible histopathologic alterations in the peritoneum of rats exposed to heat shock due to PL with saline solution at temperature of 50oC shortly followed by another PL with saline solution at temperature between 0oC and 2oC
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