Abstract
The Pacifi c oyster, Crassostrea gigas Thunberg, 1793 (2n = 20) is an economically important mollusc species cultured throughout the world. The most frequently used technique for molecular cytogenetic studies is fl uorescence in situ hybridisation which offers new opportunities for the identifi cation of oyster chromo- somes. In oysters, it has been used to locate telomeric sequences, satellite DNA, sim- ple sequence repeats, ribosomal RNA genes, and bacteriophage P1 clones. However, regarding chromosome identifi cation, no study has been done with histone H3 gene. Histone H3 is among the most conserved eukaryotic proteins. Most histone H3 genes are repeatedly organised into clusters, which make them an ideal chromosomal mark- er. In bivalves, some data exist concerning sequence information but little knowledge is available concerning the physical mapping of histone genes. The histone H3 gene was sequenced in C. gigas and phylogenetic analysis revealed that C. gigas was more closely related to Ostrea edulis Linnaeus, 1758 and species of the genus Mytilus Lin- naeus, 1758. In C. gigas, the histone H3 gene was mapped on two different pairs of chromosomes, one at an interstitial site on the long arm of chromosome pair 4, and the other on the telomeres of the smaller chromosome pair (pair 10). Polymorphism was detected on the telomeres of pair 10, once it was possible to observe single or double signals. Comparative chromosomal mapping should improve our understanding of bivalve genome organisation.
Highlights
The Pacific oyster, Crassostrea gigas Thunberg, 1793, is an economically important oyster species cultured throughout the world
Primers were aligned with the contig and unalignable regions were excluded from phylogenetic analysis
The histone H3 gene sequence from C. gigas and other histone H3 gene sequences were reduced to 305 bp for phylogenetic analysis to have a good alignment with the 41 histone H3 gene sequences selected from GenBank Database
Summary
The Pacific oyster, Crassostrea gigas Thunberg, 1793, is an economically important oyster species cultured throughout the world. We applied FISH technique with histone H3 gene probe in order to determine its location and distribution in the genome of C. gigas. Sequencing of histone H3 gene in Crassostrea gigas, sequence and phylogenetic analyses.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
