Abstract
The effect of superovulation by pregnant mare serum gonadotropin (PMSG) on autologous transplanted ovaries in the lumbar muscles of mice was histomorphometrically evaluated using the indices of number and volume of different kind of follicles and volume of corpora lutea, ovary, and stroma. Angiogenesis was observed after mouse ovarian transplantation on days 14 and 21 after ovarian grafting. After transplantation, the total number and volume of primary and secondary follicles reduced, while PMSG superovulation increased the total number and total volume of tertiary follicles and also the ovarian volume after transplantation. Transplantation increased the average size of primary, secondary, and tertiary follicles. Therefore, primary and secondary follicles can survive after autologous transplantation but their reservations diminished by increasing the time of transplantation. However, number of tertiary follicles and their response to superovulation increased over time after transplantation.
Highlights
Ovary transplantation is a method for preservation of endangered and valuable species [1]
Histomorphometric analysis showed that there was a significant reduction in the number and total volume of primary follicles in the transplantation (14 and 21 d) and pregnant mare serum gonadotropin (PMSG)/ transplantation (14 and 21 d) groups compared with the control and PMSG groups (P < 0.05, Figures 3(a) and 3(b))
There was a significant decrease in the mean primary follicle volume in the PMSG group in comparison with the other groups (P < 0.05)
Summary
Ovary transplantation is a method for preservation of endangered and valuable species [1]. Ovarian transplantation has the potential application for maintaining the fertility after chemotherapy and radiotherapy in women [2]. As a result of the ovarian transplantation, the possible depletion of follicle reserve and limitation of fertility restoration exist [3]. For evaluation of the effect of ischemia after ovarian transplantation, whole or piece of small ovaries of laboratory rodents can be used [5]. It is shown that ischemia may cause disappearance of 50% or even greater percentage of primary follicles and almost all of the growing follicles 3 to 7 days after transplantation and before development of angiogenesis [7]
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