Abstract
Objectives:This study aimed to investigate the 'Cytoprotective effect of Lawsonia inermis aqueous leaf-extract on aluminium-induced Oxidative stress in Histomorphometric of the Seminiferous tubule and Stereology of Germ Cells of adult male Wistar rats', assessing its effect on the Histomorphometry of the Seminiferous tubule and Stereology of Germ Cells.Methods:Thirty-five adult male Wistar rats, weighing between 100-196g, and fifteen mice of the same weight range were used. Lawsonia inermis extracts and aluminum chloride (AlCl3) were administered for a period of three (3) weeks, with Five (5) rats per group. Group 1 (control), received rat pellets and distilled water. Group 2 received 60mg/kg/d aqueous extract. Group 3 received 0.5mg/kg/d of AlCl3. Group 4 received 0.5mg/kg/d of AlCl3 and 60mg/kg/d of aqueous extract orally. Group 5 received 0.5mg/kg/d of AlCl3 and 75mg/kg/d of aqueous extract orally. Group 6 received 0.5mg/kg/d of AlCl3 and 100mg/kg/d of aqueous extract orally. Group 7 received 0.5mg/k/d of AlCl3 and 5mg/Kg/d of ascorbic acid orally. Twenty-four hours after the last administration, the animals were weighed, sedated with chloroform and blood was collected. The testes were removed and weighed.Results:There were statistically significant changes in the percentage of seminiferous tubular and seminiferous ductal diameter within the experimental animals in all the groups (p<0.05). Stereological findings revealed increase in spermatogonia, primary spermatocytes, round Spermatids and elongated spematids, spermatozoa, Sertoli cells population of the control rats while the rats given 0.5mg of aluminum chloride per kg of body weight had the lowest value (p<0.05).Conclusion:In this study, we demonstrated the affected histomorphometry of the seminiferous tubule and stereology of germ cells in testes, where stress impacts were most felt and subsequently translated into drastic reproductive dysfunction and distortion of spermatogenesis.
Highlights
Plant-base medicine has been wholly or partially a source of medical therapy (Trivedi, 2006) with an estimated 80% of the world population currently seeking therapeutic solution from herbal medicine as primary health care, and this has gained recognition in several nations of the world, as well as in the World Health Organization (WHO, 2018)
This study aimed to investigate the 'cytoprotective effect of Lawsonia inermis aqueous leaf-extract on aluminum-induced oxidative stress on the histomorphometry of the seminiferous tubules and the stereology of germ cells of adult male Wistar rats'
Aluminum chloride and ascorbic acid were bought in the Mich-Deson Hospital equipment store, Upper Taiwo, Ilorin
Summary
Plant-base medicine has been wholly or partially a source of medical therapy (Trivedi, 2006) with an estimated 80% of the world population currently seeking therapeutic solution from herbal medicine as primary health care, and this has gained recognition in several nations of the world, as well as in the World Health Organization (WHO, 2018). For over 9,000 years, Henna, which has the botanical name of Lawsonia inermis, has been used as the substance for drawing tattoos on the body. Apart from using Lawsonia inermis cosmetically, people use the plant as hair coloring agent in many parts of the world (Sridhar et al, 2016). 2- hydroxy-1:4 napthaquinone (C10H6O3), is a coloring agent in henna. Other constituents present are gallic acid, glucose, mannitol, fats, resin (2%), mucilage and traces of an alkaloid. Leaves yield hennatannic acid and an olive oil green resin, soluble in ether and alcohol (Pratibha & Korwar, 1999). Six compounds were identified in Lawsonia inermis leaves by GC-MS analysis, and the prevailing compounds were methyl D-Glucopyranoside (51.73%) and lawsone (19.19%) (Hema et al, 2010)
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