Histomorphological and Histochemical Study of the Uterus of the Adult Guinea Pigs (Cavica porcellus)

F.J Al-Saffar,Hazim N.H Al-Ebbadi

doi:10.17485/ijst/2019/v12i46/148620

Abstract

Objectives: This study was carried out to identify the histomorphological and histochemical aspects of the uterus in the adult guinea pig (Cavica porcellus). To conduct such project, fourteen adult guinea pigs at their diestrous period were bought from the local breeders directly. Methods: Animals were euthanized, dissected, and subsequently uteruses specimens were collected and fixed by 10% neutral buffered formalin and some specimens were fixed in Bouin’s solution for subsequent histochemical staining. Then after specimens were subjected to routine processes such as dehydration, clearing, embedding, and block preparation. Sections of 6 µm were prepared and stained with haematoxylin–eosin, Masson’s Trichrome, Alcian blue (pH 2.5), and Periodic acid shiff stains. Findings: Gross findings of the uteruses revealed duplex uterus in the guinea pigs. It possessed two separate uterine horns united caudally to form the body, suspended by mesometrium in the abdominal cavity. Caudally, body formed the cervix, which was short and projected into the vaginal cavity forming portio vaginalis uteri with one cervical orifice.Microscopic findings revealed abrupt changes in the wall of the isthmus into the uterine horn at the utero-tubal junction. The wall of the uterus showed endometrium, vascular layer, myometrium, and adventitia or serosa. Lining epithelium was simple columnar which was similarly lined the endometrial glands. Myometrium was consisted of inner circular and outer longitudinal bundles of smooth muscle fibers. In the body, the lining epithelium was replaced gradually by simple mucous columnar epithelium. In addition to that the mucosa was thrown into long folds leaving small lumen at the cervix called os cervix. Such changes were associated with endometrial glands gradual diminishing till the cervix. Histochemically, the non-ciliated columnar cells present in the body and cervix were stained positively with AB (pH 2.5) and PAS stains. The reaction with AB (pH 2.5) was more intense compared with the staining with PAS so that it indicated that mucin was more acidic than neutral in nature.Keywords: Guinea Pig, Uterus, Diestrous, Histochemistry

Highlights

Domestic guinea pigs (Cavia porcellus) were belonging to their ancestor; the wild cavy (Caviaaperea) which was considered one of the common rodents lived in the South America
In contrast to the huge number of researches conducted on the reproductive organs of domesticated animal species, few of them and paucity of works were focused on the female reproductive tract of the guinea pigs
Gross examination of the present study revealed duplex uterus in the studied guinea pigs, having two separate

Summary

Introduction

Domestic guinea pigs (Cavia porcellus) were belonging to their ancestor; the wild cavy (Caviaaperea) which was considered one of the common rodents lived in the South America. This species is herbivorous rodent characterised by their stocky body, short neck and limbs and they were more closely related to porcupines than mice and rats.[1,2]. In contrast to the huge number of researches conducted on the reproductive organs of domesticated animal species, few of them and paucity of works were focused on the female reproductive tract of the guinea pigs. Few researches were dealt with avian reproductive organs such as in pre- and post-hatched mallard.[10,11]

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