Histomorphologic characterization of the hepatopancreas of freshwater prawn Macrobrachium rosenbergii (De Man, 1879)

M.A.S Silva,M.E Almeida Neto,R.R Guerra,I.T.F Santos,B.O Ramiro

doi:10.1590/1678-4162-10497

M.A.S Silva, M.E Almeida Neto + Show 3 more

Open Access

https://doi.org/10.1590/1678-4162-10497

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Abstract

ABSTRACT This study aimed to describe the Macrobrachium rosenbergii hepatopancreas histomorphology. The hepatopancreas is constituted by a set of blind end tubules, divided into proximal, middle, and distal regions, with the epithelium formed by E, F, B, R, and M cells differently of other species. Measurements of the length and width of the tubules were 419.64+69.09µm and 117.42+16.99µm, respectively. The percentage of each cell type per region was: proximal region (40%B, 20%F, 6.7%M, 33.3%R), middle region (45.4%B, 18.2%F, 9.1%M, and 27.3%R) and distal region (36.4%E, 27.2%B, 18.2%F, 9.1%M, 9.1%R). Cell B that stores glycogen and lipids, is the most commonly found cell in proximal and middle regions. In the distal region, the E, responsible for the mitosis, is the most prominent. M, responsible by nutrient storage, is numerically constant among the portions differently in the Macrobrachium amazonicum. The study for the first time also suggests that in addition to digestive enzymes, the F cell produces protective mucus. The present study generated for the first time a morphometric profile of M. rosenbergii hepatopancreas, demonstrating differences from other species, and can be an important tool for new studies in nutrition, reproduction, and production with the species.

Highlights

Macrobrachium rosenbergii is a species originating from Malaysia that belongs to the order of the decapods (Valenti, 2001)
It could be observed that the hepatopancreas of Macrobrachium rosenbergii is constituted by a set of blind end tubules that connect to the main digestive tube and are immersed in the hemolymph
By hematoxylin and eosin stain, which basically consists in staining of basophilic and acidophilic portions of cells, it was possible to identify and differentiate the types of cells found in the epithelial tissue of the hepatopancreatic tubules

Summary

Introduction

Macrobrachium rosenbergii is a species originating from Malaysia that belongs to the order of the decapods (Valenti, 2001). The hepatopancreas receives different denominations, such as: liver, pancreas, midgut glands, gastric gland, digestive gland, anterior cecum, digestive diverticula, digestive organ, and middle intestinal gland In most crustaceans, this organ presents different levels of structural and functional complexity. Within the order of decapods, the hepatopancreas is well developed and forms a complex network of ducts and tubules in the blind end which occupies most of the cephalo-thoracic cavity (Gibson and Barker, 1979). It is involved in different functions, such as: food digestion, absorption of nutrients, storage of digestion products, mainly in the form of glycogen, and removal of residual material in the form of feces. In crustaceans, it is considered the most effective and efficient organ of the digestive system (Vicentini et al, 2009)

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