Abstract
Background: The use of eco‐friendly natural dyes has become a matter of significance due to increased environmental awareness. However, the scientific studies and systematic reports with natural dyes are insufficient. In this study, we have explored the histomorphological properties of several plant‐based dyes.Methodology: Paraffin‐embedded human tissue sections were stained with a variety of plant‐based dyes sourced from Nigeria to determine factors that produce the optimal staining efficiency in the dyes. The stained tissues were studied under bright‐field and also using a multi‐spectral imaging system to histomorphologically analyze the staining characteristics on the different tissues.Results: The staining characteristics and factors influencing staining efficiency of the different dyes on human tissues were determined. L. cyanescens stained the nuclei and cytoplasm with strong nuclear labeling in all the tissues. H. sabdariffa selectively and strongly labeled the nuclei in the tissues stained. The deep pink from H. sabdariffa and the blue from L. cyanescens gave good contrast, making a good combination for staining of tissues.Conclusion: Our study has shown L. cyanescens and H. sabdariffa as effective, cheap and readily available histological stains derived from our rich biota.
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