Abstract
The aim of this work was to analyze histologically and histometrically the sublingual gland of mice infected with the RAL strain of T. cruzi, according to the sex. Swiss mice (Mus musculus) were inoculated with 2 x 10(4) blood trypomastigotes of the RAL strain of T. cruzi. In the peak of the parasitemia (12th day) the mice were sacrificed, and the sublingual glands were fixed in ALFAC. HE-stained histological sections were evaluated histometrically. The parasitemia was higher in females. Histopatologically, acini of the infected animals were smaller, with scanty production of secretion, and smaller striated ducts. The nuclei of the demilunes were smaller and showed amastigote nests in the cytoplasm. Karyometrically, nuclei of the acini, demilunes and striated ducts were smaller in the infected mice. Stereologically, it was observed that relative volumes of acini and ducts were smaller and, inversely, relative volumen were greater for the conjunctive tissue in the infected males. The surface densities of acini and ducts were bigger and the diameter and thickness of the wall were smaller in this group. On the other hand, relative volume of acini was smaller and those of the ducts and conjunctive tissue were bigger in the infected females. The diameter and thickness of the wall of acini were smaller, and those of the striated ducts were bigger in this group. The RAL strain of T. cruzi caused general atrophy in the sublingual gland, with numerous nests of parasites in the glandular parenchyma.
Highlights
Chagas y Villela (1922) fueron los primeros que describieron la hipertrofia de las glándulas salivales, especialmente en las parótidas, de pacientes con enfermedad de Chagas portadores de mega-esófago
The aim of this work was to analyze histologically and histometrically the sublingual gland of mice infected with the RAL strain of T. cruzi, according to the sex
In the peak of the parasitemia (12th day) the mice were sacrificed, and the sublingual glands were fixed in ALFAC
Summary
Usamos 50 ratones blancos (Mus musculus), variedad Swiss (25 machos y 25 hembras), con 20 g de peso corporal medio. En los ratones machos infectados por el T. cruzi, los ácinos de las glándulas sublinguales poseían escaso producto de secreción, y, al igual que los conductos estriados eran menores que en los machos controles (Fig. 3). Histometría: el análisis cariométrico de las células de los ácinos, medialunas serosas y conductos estriados de las glándulas sublinguales mostró que la infección por la cepa RAL del T. cruzi causó una reducción estadísticamente significativa del tamaño de los núcleos en todas esas estructuras. Cuando se compararon los resultados cariométricos entre los ratones controles según el sexo, se demostró que el tamaño nuclear fue significativamente mayor en los ácinos (p
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
