Histology

Abstract

ABSTRACT I. Technical Methods.—l.A newHot-stage.—Panum (‘Nord. Medic. Arkiv.,’ viii) finds that in the hot-stages for microscopical observation now in use it is impossible to determine with accuracy the temperature of the object under observation, and there is the further inconvenience of con-sensation of water on the object. To remedy these defects he has constructed a hot chamber of tin plate, which surrounds the lower part of the microscope as well as the stage, light being admitted to the mirror through a movable pane of glass in front. The sides and back of the chamber are double, and thus form a large vessel containing water, which can be easily heated. The roof is perforated to allow the tube of the microscope to pass through, and also to admit a small thermometer, the bulb of which is placed near the object-glass. The object is introduced or manipulated through openings in the sides of the chamber, which can be closed with corks. The circulation of water enclosed in the walls of the chamber maintains a very uniform temperature; so large is the mass of heated material that the existence of small openings has little effect on the temperature of the air inside, nor is this materially affected even by opening the side or front windows.