Histology research on repairing of rabbit articular cartilage defects with naringin and tissue engineering cartilage

Abstract

At present, there are many methods to treat cartilage defects, but none radically repairs the articular cartilage defects. To histologically verify the effect of naringin combined with tissue engineering cartilage on the repair of rabbit articular cartilage defects. Rabbit bone marrow mesenchymal stem cells following in vitro proliferation were compounded onto acellular dermal matrix, which was then implanted into rabbit knee cartilage defects. Naringin was also given by lavage. Hematoxylin-eosin staining, Masson trichrome staining, toluidine blue dyeing, type II collagen staining and type X collagen staining were performed in the repaired tissue. After 8 weeks post-surgery, the defects repaired with the naringin and stem cells composite were turned into milky-white and transparent smooth tissue. The defective tissue which was repaired, was very similar to normal cartilage tissue, with smooth surface. After the histology research, we found that the defect tissue was filled with new cartilage tissue. Results indicated that naringin combined with tissue engineering cartilage can promote the repair of articular cartilage defects in rabbits. Subject headings: bone marrow; mesenchymal stem cells; dermis; tissue engineering; cartilage, articular; polypodiaceae Funding: Fund for Young Teachers by Beijing University of Chinese Medicine, No. JYBZZ-JS060 Li CG, Qu Y, Ye C, Chen J, Wang FX, Li PY, Li SH, Ren JP, Qi J. Histology research on repairing of rabbit articular cartilage defects with naringin and tissue engineering cartilage. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(20): 3165-3171.