Abstract
Survivors of Ebola virus infection may become subclinically infected, but whether animal models recapitulate this complication is unclear. Using histology in combination with immunohistochemistry and in situ hybridization in a retrospective review of a guinea pig confirmation-of-virulence study, we demonstrate for the first time Ebola virus infection in hepatic oval cells, the endocardium and stroma of the atrioventricular valves and chordae tendinae, satellite cells of peripheral ganglia, neurofibroblasts and Schwann cells of peripheral nerves and ganglia, smooth muscle cells of the uterine myometrium and vaginal wall, acini of the parotid salivary glands, thyroid follicular cells, adrenal medullary cells, pancreatic islet cells, endometrial glandular and surface epithelium, and the epithelium of the vagina, penis and, prepuce. These findings indicate that standard animal models for Ebola virus disease are not as well-described as previously thought and may serve as a stepping stone for future identification of potential sites of virus persistence.
Highlights
Survivors of Ebola virus infection may become subclinically infected, but whether animal models recapitulate this complication is unclear
Ebola virus disease (EVD) is a severe and frequently lethal affliction of humans caused by infection with any of three members of the mononegavirus family Filoviridae: Bundibugyo virus (BDBV), Ebola virus (EBOV), and Sudan virus (SUDV)
Long term sequelae in individual survivors of acute EVD and the similar Marburg virus disease (MVD) and filovirus persistence followed by disease relapse or sexual transmission had been reported before this outbreak[3,4,5,6,7,8]
Summary
Survivors of Ebola virus infection may become subclinically infected, but whether animal models recapitulate this complication is unclear. Using histology in combination with immunohistochemistry and in situ hybridization in a retrospective review of a guinea pig confirmation-of-virulence study, we demonstrate for the first time Ebola virus infection in hepatic oval cells, the endocardium and stroma of the atrioventricular valves and chordae tendinae, satellite cells of peripheral ganglia, neurofibroblasts and Schwann cells of peripheral nerves and ganglia, smooth muscle cells of the uterine myometrium and vaginal wall, acini of the parotid salivary glands, thyroid follicular cells, adrenal medullary cells, pancreatic islet cells, endometrial glandular and surface epithelium, and the epithelium of the vagina, penis and, prepuce These findings indicate that standard animal models for Ebola virus disease are not as well-described as previously thought and may serve as a stepping stone for future identification of potential sites of virus persistence. In a retrospective histologic review of tissues from EBOV-infected guinea pigs, in combination with immunohistochemistry and in situ hybridization, we demonstrate that EBOV infection of several tissues and numerous cell types has been overlooked
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