Histological study of the effect of sildenafil citrate on ovulation induction in mature female mice

Abstract

The aim of the present study is to investigate the effects of oral administration with different concentrations of sildenafil citrate (SC) on some of the reproductive parameters as ovulation induction in the female mice. In the experimental work, thirty of healthy female mice at the age (10-12) weeks with weight (23-25) gram were included in this study. They were divided in three groups (10 mature females’ mice/group) including one group as control according to different doses of sildenafil citrate (0.5 mg/kg, 1 mg/kg) throughout administration period for 6 days. Parameters were assessed involving study the histological changes in ovary as, number each of primary follicles, growing follicles, graafian follicles, evaluated using hematoxyline and eosin stains. The results of the present study showed a significant improvement (P<0.05) in the reproductive parameters using both concentrations (0.5 and 1mg/ kg) of the sildenafil citrate when compared with the control group as; number each of primary follicles, growing follicles and grafian follicles.Moreover, high concentration of (1mg/kg), sildenafil citrate showed a significant enhancement in the reproductive parameters which were mentioned previously compare with low dose of sildenafil citrate (0.5mg/kg) and control group .Therefore, it was concluded that the sildenafil citrate supplementation may have important role on the ovulation induction in females mice.