HISTOLOGICAL STUDY AND FUNCTIONAL EVALUATION OF CHONDROCYTES IN TRANSGENIC MICE WITH POINT MUTATION OF COL2A1 GENE

Abstract

Angiotensin-converting enzyme (ACE) inhibitors are widely used in treatment of heart failure, but little is known regarding whether ACE inhibitors regulate the activity of matrix metalloproteinases (MMPs) and the tissue inhibitor of MMPs (TIMPs) in cardiac cells. The purpose of this study was to determine the ability and possible signal pathway involved of imidaprilat, an ACE inhibitor, to modulate MMP-2 and TIMP-2 in human cardiac fibroblasts in the presence of interleukin (IL)-1β.Using gelatin zymography and RT-PCR and Griess analysis,we found that IL-1β increased the MMP-2 activity and transcription and nitric oxide(NO) production from supernatant of culture medium.These effects of IL-1β were inhibited by imidaprilat or the NO synthase inhibitor, L-NMMA. Sodium nitroprusside (SNP), an exogenous NO donor, prevented significantly the effects of imidaprilat on MMP-2 inhibition. Imidaprilat alone didn't affect MMP-2 activity and expression. Neither IL-1β nor imidaprilat has no effect on TIMP-2 transcription in cardiac fibroblasts.The current study demonstrates imidaprilat inhibits MMP-2 activity and expression in human cardiac fibroblasts induced by IL-1β via NO-dependent pathway. These data suggest that the beneficial effect of ACE inhibitors against left cardiac remodeling and heart failure may be due at least in part to regulating MMPs activity and expression by modulation of NO pathway.