HISTOLOGICAL OF MICE MAMMAL GLANDS THAT SUPPLEMENTATION WITH INFUSE WATER OF MANIHOT ESCULENTA LEAVES

Abstract

Phytoestrogens are plant compounds whose structure and properties are similar to estrogens. Flavonoids are one of the phytoestrogen compounds and cassava leaves are known to contain flavonoid compounds. This study was conducted to determine the effect of steeping cassava leaves on the histology of the mammary glands of female mice. This study used a completely randomized design (CRD) which consisted of 4 treatments and 6 replications. The treatment consisted of K0 as a control given 0.5 mL/bb/day of distilled water, P1 given 0.5 mL of cassava leaves steeped with a concentration of 7%, P2 given 0.5 mL of cassava leaves steeped at a concentration of 9%, P3 which was given a decoction of cassava leaves 0.5 mL with a concentration of 11% for 21 days. On the 22nd day, surgery was performed, then the mammary glands of the mice were taken to make histological incisions. Histological incisions of the mice's mammary glands were made using the paraffin method and hematoxylyn-eosin staining and observed under a microscope with 100x magnification with the help of optilab and the image raster program. Parameters observed included the number of mammary gland alveoli, the number of mammary gland ducts, and the diameter of the mammary gland ducts. The data obtained was analyzed statistically with the SPSS series 23 for Windows program. If the results of the analysis show a significant difference, then continue with the Duncan test. The results showed that steeping cassava leaves significantly increased the average number of alveoli, number of ducts and duct diameter of the mammary glands of female mice. The most influential dose of steeped cassava leaves was the dose of 9 g/Kg BW of female mice.