Histological observation and occurrence of apoptosis in the gill from estuary Amazon fish

Abstract

Event Abstract Back to Event Histological observation and occurrence of apoptosis in the gill from estuary Amazon fish Leonardo F. Paixão1, Luiz Fernando S. Oliveira1, Zélia M. Nunes2, Rossineide M. Rocha1 and Maria Auxiliadora P. Ferreira1* 1 UNIVERSIDADE FEDERAL DO PARÁ, Histologia e Embriologia, Brazil 2 Universidade Federal do Pará, Campus de Bragança, Brazil Sciades herszbergii is a fish species commomly know as "blue catfish" and is one the most important natural resources an estuary in the Amazon region. In this study histological observations and apoptosis analysis was made in a gill tissue of the S. herszbergii from two environments: I, away from pollution sources and; II, waste from industry, the last one was used to relate the health status of the fishes in the environmental. Gill fragments were fixed 4% paraformaldehyde solution for 24 h and submitted for histological routine processing for embedding in paraffin. Sections of 7 μm were stained with Hematoxylin-Eosin for analysis under light microscopy. For immunohistochemycal analyses gill sections were incubated in the primary polyclonal antibody anti-caspase 3, made in rabbit. Slides were revealed using 3,3'-diaminobenzidine. The results showed the type and level of lesions were verified between two study sites. A variety of severe damages were observed in specimens including aneurysms, epithelial lifting, and intense hyperplasia, specially on the site II. Apoptosis analyses also revealed injuries in the gill tissue of specimens from these two sites. While on site I only few specimens showed mild changes in gill tissues and rare apoptosis marking. These results suggest the gill histological and immunohistochemical analysis are a good biomarker, and the specie S. herszbergii could be used as a bioindicator for environmental monitoring. References Abdel-Moneim, A. M.; Al-Kahtani, M. A. and Elmenshawy, O. M. (2012). Histopathological biomarkers in gills and liver of Oreochromis niloticus from polluted wetland environments, Saudi Arabia. Chemosphere, 88(8): 1028-1035. doi:10.1016/j.chemosphere.2012.04.001 Dabrowska, H., Ostaszewska, T., Kamaszewski, M., Antoniak, A., Napora-Rutkowski, Ł., Kopko, O., Lang, T., Fricke, N. F., Lehtonen, K. K., (2012). Histopathological, histomorphometrical, and immunohistochemical biomarkers in flounder (Platichthys flesus) from the southern Baltic Sea. Ecotoxicol. Environ. Saf. 78, 14–21. doi: 10.1016/j.ecoenv.2011.10.025 Nascimento, A.A.; Araújo, F.G.; Gomes, I.D.; Mendes, R.M.M.; Sales, A. (2012). Fish gills alterations as potential biomarkers of environmental quality in a eutrophized tropical river in South-Eastern Brazil. Anat. Histol. Embryol. 41(3): 209-216. doi:10.1111/j.1439-0264.2011.01125.x Keywords: Apoptosis, gill, fish, biomarkers, estuary Amazon Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Poster Presentation Topic: Physiology, Behavior and Toxicology Citation: Paixão LF, Oliveira LS, Nunes ZM, Rocha RM and Ferreira MP (2015). Histological observation and occurrence of apoptosis in the gill from estuary Amazon fish. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.fmars.2015.03.00100 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Nov 2015; Published Online: 21 Nov 2015. * Correspondence: Dr. Maria Auxiliadora P Ferreira, UNIVERSIDADE FEDERAL DO PARÁ, Histologia e Embriologia, Belem, Pará, +55, Brazil, auxi@ufpa.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Leonardo F Paixão Luiz Fernando S Oliveira Zélia M Nunes Rossineide M Rocha Maria Auxiliadora P Ferreira Google Leonardo F Paixão Luiz Fernando S Oliveira Zélia M Nunes Rossineide M Rocha Maria Auxiliadora P Ferreira Google Scholar Leonardo F Paixão Luiz Fernando S Oliveira Zélia M Nunes Rossineide M Rocha Maria Auxiliadora P Ferreira PubMed Leonardo F Paixão Luiz Fernando S Oliveira Zélia M Nunes Rossineide M Rocha Maria Auxiliadora P Ferreira Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.