Abstract

Abstract The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits’ teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn’s post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application.

Highlights

  • The best organized newly formed calcified tissue was seen after hydroxyapatite with calcium silicate cement (HA-CS) application

  • Calcium silicate cements are superior to most endodontic materials, taking into account their biocompatibility, bioactivity and sealing properties

  • Good biological properties of calcium silicate cements are related to calcium hydroxide, which is released during the material setting and stimulates the proliferation and differentiation of different cells response for tissue repair [3,4,5]

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Summary

INTRODUCTION

Calcium silicate cements are superior to most endodontic materials, taking into account their biocompatibility, bioactivity and sealing properties. The first generation of commercial calcium silicate cements known as MTAs cements, has a long initial setting time (3h), as a result of their chemical composition and hygroscopic nature [6] and contain a trace of heavy metals as a result of the manufacturing by purifying and modification of Portland cement [7] These cements usually contain bismuth oxide as radiopacifier, which interferes with the hydration processes of cement [8] and may react with dentin collagen resulting in tooth staining [9]. Initial in vitro testing of CS and HA-CS has shown the absence of toxic effects on human cells in terms of genotoxicity [18] Implantation of these cements in subcutaneous rat tissue or as pulp capping materials in rabbits’ teeth resulted in minimal inflammatory response, confirming their biocompatibility [19, 20]. The aim of this study was to evaluate periradicular tissue inflammatory reactions and the formation of calcified tissue after implantation of CS and HA-CS into root canals of rabbits’ teeth

MATERIALS AND METHODS
Experimental procedure
Histological procedure and histological analysis
RESULTS
DISCUSSION
CONCLUSION
Camilleri J
34. Anselme K
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