Histological Effects of Sesamum Indicum Seeds on Mammary Gland tissue in Female white Rats

Abstract

This study was conducted to investigate the effects of sesame seed on the growth and development of mammary glands in white females rats during the stages of (virginity, pregnancy and lactating), where the effect of 30% sesame seed on the growth and development of mammary glands was studied for 20 days. Sixty-three female Albino rats were randomly divided into three groups (virgins, pregnant and lactating) 12 rats / for each group, the three main groups were subdivided into secondary groups (control and treatment) with 6 rats / for each group. Then toe parameters were conducted including: Histological and histochemical studies for each group and through these studies the following results were obtained:Histological specimens stain with hematoxylin and eosin stains revealed that the virgins treated with sesame seeds showed an increase in the number of the lobules filled with large numbers of expansive alveoli compared to the control group. In the pregnant groups, the lobules filled with alveoli were observed in larger numbers and diameters. In the lactation groups, the lobules are more numerous and the alveolus were more expansive and numerous in comparison with the control group.The histochemical studies of the Periodic Acid Schiff (PAS) stain showed a positive intra-alveolar reaction in the mammary glands tissue for each animal of treated and control groups, except the virginity control group which showed a negative reaction to the stain. Whereas the Trichrome stain, which characterized only the control group of virgins with more density of collagen fibers compared to the rest of the groups which were characterized by a deficiency in the density collagen fiber groups for all stages compared to the control groups.From the current study it can be concluded that the sesame seeds have an effective effect on the growth and development of mammary gland tissue