Histological Effects of Nigella Sativa on Aspirin-Induced Nephrotoxicity in Albino Rats.

Abstract

To determine the histological effects of Nigella sativa on kidneys against aspirin-induced nephrotoxicity in albino rats. An experimental study. Anatomy Department, University of Health Sciences, Lahore, from January 2014 to December 2015. Thirty-two rats were equally divided into four groups A, B, C and D of eight animals each. Group A (control) was provided with single oral dose of 10 mg/100 gm body weight of 1% methyl cellulose. Group B (experimental group) and C (self recovery group) were provided with single oral dose of 1000 mg/kg aspirin. Group D was provided with 250 mg/kg ethanolic extract of Nigella sativa followed by 1000 mg/kg aspirin by oral gavage, after that only extract of Nigella sativa was given for seven days. Animals of groups A, C and D were sacrificed on the 8th day of experiment and that of group B on the second day of experiment. Kidneys were excised and fixed in 10% formalin solution. Hematoxylin, eosin and periodic acid Schiff's reagents were used to stain the kidney tissues. Histological slides were prepared to study proximal convoluted tubules. Histological parameters were normal in control group A. Significant impairment was present in group B. There was no self-recovery in group C. Significant improvement was present in all the parameters of group D. Nigella sativa has protective effect on aspirin-induced nephrotoxicity in rats.