Abstract
BackgroundLateral root constitutes an important part of root system either in tap root plants or fibrous root plants. The development of lateral root primordium (LRP) in Arabidopsis, which has a tap root system with simple radial structure of primary root, has been well characterized. However, limited knowledge has been acquired on the plants with fibrous root system, such as rice. This is mainly due to their multiple cell layers coated on root, which disturb the observation of LRP.ResultsWe used an easy and quick method to strip the epidermal and cortex tissues of primary root so that the LRP can be easily observed under microscope. In this way, we observed the developmental processes of LRP in rice. In addition, we described the expression dynamics of several root development related genes, especially OsPINs (PIN-FORWMED), during the process of LRP development.ConclusionsWe reported an easy and quick method for LRP observation in rice and suggested a “fountain” model of auxin transport in LRP of rice, which is similar with that in Arabidopsis.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-014-0042-x) contains supplementary material, which is available to authorized users.
Highlights
Lateral root constitutes an important part of root system either in tap root plants or fibrous root plants
Because of the epidermal and the ground tissues, which are composed of exodermis, sclerenchyma, cortex and endodermis, heavily cover the lateral root primordium (LRP) on root of rice (Rebouillat et al 2009), it is difficult to clearly observe the LRP through microscope (Figure 1A)
In Arabidopsis, the LRPs are allocated along the parent root in a regular left-right alternating pattern (De Smet et al 2007; Moreno-Risueno et al 2010)
Summary
We used an easy and quick method to strip the epidermal and cortex tissues of primary root so that the LRP can be observed under microscope. In this way, we observed the developmental processes of LRP in rice. We described the expression dynamics of several root development related genes, especially OsPINs (PIN-FORWMED), during the process of LRP development. Conclusions: We reported an easy and quick method for LRP observation in rice and suggested a “fountain” model of auxin transport in LRP of rice, which is similar with that in Arabidopsis
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