Histological changes of the dermal-epidermal junction of superficial scarin rabbit ears induced by ultrapulse CO2 fractional laser

Abstract

Objective To explore the effects of wound healing and histological changes by utilizing ultrapulse CO2 fractional laser for the treatment of superficial scar in dermal-epidermal junction (DEJ) in rabbit ears. Methods We adopted traditional iodophor treatment and moist exposed burn ointment treatment repairing the wound and utilizing ultra pulse CO2 fractional laser for the treatment of rabbit ears superficial scar. The superficial scars in rabbit ear were induced with ultra pulse CO2 fractional laser. In 1, 2, 4, 8 weeks tissue samples would be taken. Using electron microscopy we observed DEJ ultrastructure and light microscopy to observe the histological changes of the dermal papilla and PAS staining for epidermis basement membrane. Results The wound healing time of moist exposed burn ointment group was shorter than that of iodophor group (P<0.05). The number of hemidesmosomes and anchoring fibers in the moist exposed burn ointment group on the 8th week was more than the 1th week, The number of hemidesmosomes and anchoring fibers in the iodophor group on 8th week was more than 1th week (P<0.05). The number of hemidesmosomes and anchoring fibers in the iodophor group was less than the moist exposed burn ointment group (P<0.05). Conclusions Ultrapulse CO2 fractional laser can cause ultra structural changes in scarring dermal-epidermal junction area, wet wound healing environment promotes the reconstruction of DEJ district organization, and rebuilding the function of the scar skin has the positive significance. Key words: Ultrapulse CO2 Fractional laser; Superficial scar; Dermal-epidermal junction; Regeneration and reconstruction