Histological Changes Indused by Monosodium Glutamate on the Growth and Development of the Offsprings Albino Mice Cornea

Abstract

This research aim to study the effect of different doses of a Monosodium Glutamate (MSG) to cause histopathological changes in the offspring corneal for the ages 7, 15, 21, 30, and 60 days. The mothers, type Mus musculus mice has been orally administrated from 17th day of pregnancy by different concentrations (2, 4, and 8 g/Kg/ body weight) of MSG using stomach tube. Microscopic examination showed no changes in the cornea for a seventh day and administrated concentrations of 2 and 4 g / kg body weight, but observed degeneration of fibre stroma, necrosis and the reduction of the anterior epithelial layer of the cornea at dose of 8 g / kg. In the group of 15 days, these changes are mentioned; hyperplasia in the anterior epithelium of the peripheral cornea of 2 g, deformation in the peripheral part of the cornea and the reduction of thickness and intensify the stroma components at a concentration of 4 g either at a concentration of 8 g / kg has appeared hyperplasia in fibroblast cells in the corneal stroma and wave of the basement membrane of anterior epithelial tissue of central cornea. In a group 21days, these changes are shown; necrosis and rarefaction in stroma of central cornea while the reduction of the anterior epithelial layer of the peripheral cornea notes and wave anterior surface of the cornea in the concentration of 2 g / kg. Deformation appeared and reduction layers and irregular with dissociation in the stromal fibers in some areas in the concentration of 4 g / kg, while in the concentration of 8 g / kg show damage in the anterior part of epithelial of the central corneal tissue. In a group 30 days and concentration of 2 g / kg, these changes were observed; necrosis, swelling in some anterior epithelial cells, condensation of nuclei in endothelial cells, with Keratinized at the surface of the cornea and edema in the stroma. Bulge appeared in the stroma of peripheral cornea and hyperplasia in fiberoblast at a concentration of 4g/kg, meanwhile at a concentration of 8 g/kg show necrosis under the anterior epithelium of central cornea and dissociation of stromal fiberoblast. In the group 60 Days at the concentration of 2 g / kg show damage in the anterior epithelial tissue of the peripheral cornea and hyperplasia in some parts of the anterior epithelial tissue in the central cornea. At the concentration of 4g/kg show damage in the stromal fibers of peripheral corneal epithelium and detachment of endothelial tissue. In concentration 8 g/kg reduction appeared in the anterior epithelial tissue in the certain part of the cornea, hyperplasia in endothelial tissue, condensed layer appeared on the surface of anterior epithelial tissue.