Histological Assessment and Antimicrobial Investigation of Pure Compound; 3,5,6,7- Tetrahydroxy-19-Vouacanoic Acid;(3β, 5α, 6β, 7β)-form,6,7-Dibenzoyl (Pulcherrimin A) Isolated from Caesalpinia pulcherrima Stem Bark

Abstract

This study was aimed to investigate the histological effect and antimicrobial activity of a compound [3,5,6,7-tetrahydroxy-19-vouacanoic acid;(3β, 5α, 6β, 7β)-form,6,7-dibenzoyl] previously isolated from Caesalpinia pulcherrima stem bark prior to this study. Different doses (2, 4 and 8 mg/kg) of the compound was administered to Wistar rats (both sexes) for a 28-day period. After the 28-day administration, vital organs (liver, heart, kidney, lung, uterus and testes) were harvested from the animals to ascertain their organ weight variations and histology. Microbial activity of the compound was investigated (using zone of inhibition test and minimum inhibitory concentration) for the microorganisms (Bacillus subtilis, Klebsiella pneumoniae, P. aeruginosa, Staphylococcus aureus, Candida. albican and Trychophyton rubrum). The observed weight changes in organs for all treated groups in relative to control was not significant at p < 0.05 and no observed toxic abnormalities for all organs in treated groups upon their gross examination in relative to control. Highest zone inhibition was observed at 100 mg/kg for all tested microorganisms (B. subtilis, K. pneumonia, P. aeruginosa, S. aureus, C. albican and T. rubrum). with their corresponding MICs 11, 13, 16, 33, 30 and 31 mg/ml respectively. This study suggeststhat the compound could be a potential candidate in view of developing new antimicrobial agent and potential novel drug as a vasodilator.