Abstract
BackgroundRegarding postmortem estimation, the evaluation of early histological and miRNA molecular changes have been of great concerns among forensic scientists due to uncertainty of known conventional methods. The histological and miRNA molecular changes in incisional wound remain poorly described.PurposeThis evaluation presents a histo-molecular approach that could be used to estimate the early post mortem interval in incisional wound.MethodsThe histological and miRNA (205 and 21) changes were evaluated in 18 female albino rats with incisional wound at three interval times: 0, 24, and 48 h after infliction.ResultsThe wound margin showed progressive morphological changes with increasing postmortem interval. Inflammatory phase had appeared immediately after infliction. After this period, degenerative changes began. mRNA-205 and 21 expressions, detected via real-time PCR, were increased significantly to reach their higher levels at 24 h after wound infliction, then they were significantly diminished with increasing postmortem interval.ConclusionsOur results suggest that histological and miRNA-205 and miRNA-21 changes, occurred at perimortal incisional wound, could be helpful in forensic work regarding postmortem estimation.
Highlights
Regarding postmortem estimation, the evaluation of early histological and Micro ribonucleic acid (miRNA) molecular changes have been of great concerns among forensic scientists due to uncertainty of known conventional methods
This study was conducted on 18 female albino rats with incisional wound
Forensic scientists could use these changes to evaluate the effect of the time passed since death in the incisional wounds (Gunn et al 2014; Lv et al 2014)
Summary
The evaluation of early histological and miRNA molecular changes have been of great concerns among forensic scientists due to uncertainty of known conventional methods. The histological and miRNA molecular changes in incisional wound remain poorly described. Purpose: This evaluation presents a histo-molecular approach that could be used to estimate the early post mortem interval in incisional wound. Incisional wounds have been and will remain a major cause of violent death. The nature of the wound, as ante-mortem or postmortem, have to be ascertained by the forensic pathologist (Karlsson 1998; Siddiqui et al 2016). Determination of wound vitality in perimortal injuries, and the interpretation of the chronological sequence of events is an important part in forensic work (Obac et al 2011). Ante-mortem wound shows tissue reaction and consists essentially of an acute inflammation. Many biochemical molecules (e.g., miRNAs) and cells (e.g., neutrophil and mononuclear cell) are involved in this inflammatory process (Vinay et al 2017)
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