Histological and Immunohistochemical Study on the Effect of Bisphenol A on the Retina of Female Albino Rat

Abstract

Background: Bisphenol A (BPA) is a well-known endocrine disruptor used to manufacture polycarbonate plastics and epoxy resins. Exposure of rats to low doses of BPA results in histopathological effects in their retina. Objectives: We used histological and immunohistlogical techniques for determining retina pathological changes in response to low doses of BPA and the modulating effect of both vitamin A and stem enhancer. Methods: Twenty female albino rats orally administered with 20mg BPA /kgb.wt/day for 45 days and then divided to groups and treated with vitamin A and stem enhancer. Both eyes were examined histologically and immunohistochemicaly to determine the histopathological changes in retinal layers. Results: A remarkable degenerative histopathological changes in the ganglion cell layer and inner nuclear layer appeared with bis phenol treated rats and a clear improvement was seen after treatment with both vitamin A and stem cell enhancer. Conclusions: Both of vitamin A and stem enhancer have ameliorative effect on the degenerative changes in the retinal layers and damaged estrogen receptors by the action of bisphenol A.