Histological and immunohistochemical study of the effects of tramadol administration on the ovaries of adult albino rats and the possible recovery after its Withdrawal.

Abstract

Background: Tramadol hydrochloride is a centrally active synthetic opioid. Tramadol is known to be highly effective in the treatment of the pain.Aim of the Work: The present work was conducted to outline the deleterious effects of tramadol administration on the ovaries of adult albino rats and the possible recovery after the tramadol withdrawal.Materials and Methods: Thirty adult virgin female albino rats were used in this study. They were classified into two groups; control group and experimental group. The experimental group is further divided into treated and withdrawal. Both the treated and the withdrawal groups were subcutaneously injected with Amadole in a dose of 40 mg/kg body weight three consecutive times weekly for 8 weeks while the withdrawal group was kept for another 8 weeks without treatment for the detection of the possible recovery. The rats were sacrificed. The ovaries were processed for histological and immunohistochemically study. Morphometric study was done in addition.Results: In the treated group, the ovaries had degenerated ovarian follicles with loss of their normal architecture and some atretic follicles could be detected. The secondary follicles showed primary oocytes surrounded by granulosa cells. The surrounding granulosa cells appeared swollen, disrupted, irregular with degenerated vacuolated cytoplasm. The cortical blood vessels were dilated and congested. The withdrawal group revealed degenerative changes that were comparable to the treated group.Conclusion: Tramadol induced deleterious effects on The structure of the ovary .These impairment were not improved with the withdrawal of tramadol.