HISTOLOGICAL AND HISTOCHEMICAL STUDY OF BRUNNER'S GLANDS IN LOCAL ADULT COWS

Abstract

The study was conducted to add information to the database for concentrate on theBrunner's glands of the duodenum in adult local cows. The histological study showedthat Brunner's glands present in the tunica submucosa of the duodenum in local adultcows, the glands were branched tubuloalveolar secreting mucous, extended from theend of the pylorus to the first and middle third of duodenum, and absent at the lastthird of the duodenum. The excretory ducts of Brunner's glands opened at the cryptsof lieberkuhn at the base of intestinal villi penetrating the muscularis mucosa layer.The statistical analysis showed there was a significant variation in the diameter andlumen of Brunner's glands between the first and middle third of duodenum at (P≤0.05). The diameter and lumen of Brunner's glands at the first third of duodenum werelower than that of the middle third of duodenum. The statistical study showed , thatthere was no significant variation of the lining epithelium of Brunner's glands betweenfirst and middle third of duodenum, and there was no significant variation in thethickness of Brunner's glands aggregation between first and middle third of theduodenum.The histochemical study revealed that there were different types of glycoprotein inBrunner's glands and excretory duct in local adult cows, at first third of duodenumBrunner's glands contain a large amount of neutral glycoprotein and little amount ofacidic glycoprotein, while at middle third of duodenum the Brunner's glands containthe same amount of neutral and acidic glycoprotein. At the first third of duodenum,the excretory ducts of Brunner's glands contain neutral glycoprotein more than acidicglycoprotein, while at the middle third of duodenum the excretory ducts of Brunner's glands contain a large amount of acidic glycoprotein and little amount of neutralglycoprotein.