Histological and histochemical studies on the lingual, preglottal and laryngeal salivary glands of the Japanese quail (Coturnix coturnix japonica) at the post-hatching period.

Abstract

The postnatal development and histochemistry of mucins of the lingual, preglottal and laryngeal glands in the quails were investigated by means of light microscopy using specific staining for complex carbohydrates. In this study, the tongues were taken from female and male quails from day 1 to day 60 after hatching. The salivary glands in quail's tongue comprised the lingual gland, with lateral and medial (paraentoglossal gland) portions that differ in morphology and histochemical staining, and the preglottal gland, with two lateral portions and one medial portion. The medial portion of the preglottal gland, which extended to the row of the laryngeal papillae on each side of the glottis, was described as the laryngeal gland. The salivary glands were present at hatching and their cells were functionally mature and secreted mucins. In quail of all ages, the histochemical reactions revealed that the cytoplasms of the secretory cells of the preglottal, laryngeal and paraentoglossal gland (medial portion of lingual gland) contained sialomucins and weakly sulphated epithelial mucins. Neutral mucins were absent in the paraentoglossal gland, while a small amount of neutral mucins was present in other glands. The mucins with vicinal diol groups, sialomucins and weakly sulphated epithelial mucins were mixed within the secretory cells of all the glands. All the histochemical reactions were restricted to the supranuclear regions of the secretory cells within the lateral portion of the lingual gland. In conclusion, the contents of mucins in the lingual, preglottal and laryngeal glands varied between different age groups, however, no differences in the glands' histochemistry between male and female quails were observed.