Abstract
The present study was carried out to investigate the histological and histochemical changes in the liver and skin on different developmental stages of Egyptian toad Bufo regularis to be used as a histological key for such species. Our experiment started when tadpoles began to feed. The adapted embryos are divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Both histological and histochemical results showed that the general architecture of the different organs was correlated with the state of development, i.e. larval, met-amorphic and post-metamorphic. They, therefore, displayed different characteristic features de-pending on the investigated developmental stage starting from the larval stage (stage 44) and ending with the post-metamorphic stage 66.
Highlights
Histological changes have been widely used as biomarkers, both in the laboratory [1] [2], and field studies [3]
For the transmitted electron microscope (TEM), tadpoles were fixed in 5% phosphate buffered glutaraldehyde for 8 hours and for molecular preparations tadpoles were deep frozen at −80 ̊C
1) Stage 44 The liver sections of tadpole at this stage showed a continuous mass of hepatocytes with almost centrally located nuclei
Summary
Histological changes have been widely used as biomarkers, both in the laboratory [1] [2], and field studies [3]. One of the great advantages of using histological biomarkers in environmental monitoring is that this category of biomarkers allows examining specific target organs, including gills, kidney and liver, which are responsible for vital functions, such as respiration, excretion and the accumulation and biotransformation [4]. It plays important roles in the conversion of glucose to glycogen, regulation of lipids and deamination of amino acids. It is the organ in which nutrients absorbed in the digestive tract are processed and stored for use by other parts of the body. Liver is a target of cytological modifications caused by changes in the lifestyle, once the aquatic omnivorous tadpole changes to a terrestrial carnivorous adult [5]
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