Histological and Histochemical investigation of the development of the New -Zealand rabbit’s gastric glands

Shehata Mohamed Mohamed Soliman ,Marwa M Hussein,Abdel‐Razik H Abdel‐Razik ,Omima M M Rashad1

doi:10.21608/jvmr.2020.87551

Shehata Mohamed Mohamed Soliman , Marwa M Hussein + Show 2 more

Open Access

https://doi.org/10.21608/jvmr.2020.87551

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Abstract

The present study aimed to provide a detailed description of the normal development of rabbit stomach and focusing on the histogenesis of gastric glands. In a total, 24 New Zealand White rabbit fetuses were collected at gestational days 21, 25, and 29. The stomachs of the collected fetuses were fixed in 10% neutral buffered formalin and prepared by paraffin technique then stained with Harris's Haematoxylin and Eosin, Masson's Trichrome stain, Orcein, Periodic acid-Schiff, Alcian blue, and Bromophenol blue stains. The results revealed that, at 21stgestational day, the different parts of the stomach including, cardia, fundus and pylorus could be easily distinguished. On 25th developmental day, the gastric mucosal folds were more prominent in the cardia than fundus and pylorus. At 29th developmental day, tunica mucosa and tunica submucosa of the fetal stomach were laid in longitudinally oriented folds known as rugae. The gastric gland in this age became well developed containing well-demarcated oxyntic and peptic cells. In conclusion, the rabbit stomach is completely differentiated during the embryonic life and the gastric glands were functionally active.

Highlights

The rabbit is a widely distributed animal species used for economical and laboratory purposes (Hristov et al, 2006)
It was observed that the thickness of the gastric mucosa was greater in the cardiac region and became lesser in the fundus, body and pyloric regions (Fig. 1 A-D-G)
The gastric mucosa consisted of lamina epithelialis, lamina propria, and muscularis mucosa

Summary

Introduction

The rabbit is a widely distributed animal species used for economical and laboratory purposes (Hristov et al, 2006) It is used in laboratory investigations because it is an ideal experimental model (Ghoshal and Bal, 1989). The same authors added that the folds possess cellular cords projecting downwards into the underlying connective tissue The majority of these cords appear solid with no lumen. 2017 and Kammeraad, 1942, in rat fetuses recorded that, the gastric pits are firstly noted on the 15th day of gestation. On the 24th gestational day of rabbit fetuses, the gastric epithelium has a simple columnar structure and the parietal cells occur between the bases of the epithelial cells of the gastric glands (Yaman & Girgin, 2001; Hayward, 1967and Menzies, 1958). The gastric epithelium contains well-defined glands containing parietal and mucus- secreting cells (Hayward, 1967, in rabbit and Yeomans et al, 1976 and Helander, 1969a in rat)

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