Abstract
Sixty-four of female quails, twenty-two weeks old, were used in the current study to evaluate the potential of volatile oil from garlic extract in improving the histological liver of the quails. Volatile oil isolated from garlic by using destilated technique. This study was designed according to completely randomized design, consisted of four treatment groups and six replications. Each treatment group consisted of 16 quails, with supplemental volatile oil (VO) by orally, as follow L0=without of VO; L1= VO of 75 mL; L2= VO of 100 mL and L3= VO of 125 mL. Liver tissue samples were collected using a fixative solution and the liver samples were used to determine the liver histological parameters (necrosis, apoptosis, fat degeneration, hydropic degeneration, acute inflammation, hemorrhagic) by a Mallory-asan technique dan binocular microscope. The current study showed that overall results indicated a significant treatment effect (P<0,05) for the liver histological condition. In conclusion, VO resulted in supplemented levels that significantly raised protein and lipid anabolic and induced hormone signal related growth liver tissue
Highlights
Enam puluh empat ekor puyuh berjenis kelamin betina, berumur 22 minggu telah digunakan dalam penelitian ini untuk mengkaji pengaruh pemberian minyak atsiri terhadap kondisi histologi liver puyuh
The current study showed that overall results indicated a significant treatment effect (P
Mechanism of inhibition of chemical toxicity and carcinogenesis by diallyl sulfide (DAS) and relate compounds from garlic
Summary
Enam puluh empat ekor puyuh berjenis kelamin betina, berumur 22 minggu dengan kisaran berat badan 121±4,63 g, telah digunakan dalam penelitian ini untuk mengkaji dampak pemberian minyak atsiri terhadap histologi livernya. Sampel ternak puyuh percobaan dibagi kedalam empat kelompok percobaaan, masingmasing kelompok terdiri dari 16 ekor. Pemisahan senyawa-senyawa minyak atsiri dari bawang putih dilakukan berdasarkan Block (1985), dengan prosedur, sampel bawang putih diiris dan dimasukkan ke labu alas bulat kemudian tambahkan akuades sampai seluruh sampel terendam sempurna di dalam labu alas bulat berleher panjang yang telah dirangkai dalam perangkat alat destilasi. Destilasi dilakukan bertahap pertama dengan temperatur 75-800C dan tahap berikutnya selama 4-5 jam pada temperatur 1000C (Block, 1985). Pemberian minyak atsiri pada tiap-tiap ekor puyuh percobaan dilakukan pada setiap pagi hari (jam 06.00 – 07.00) selama satu bulan, sebelum ternak diberi makan. Pemberian minyak atsiri pada ternak percobaan tersebut dilakukan dengan teknik pencekokan, menggunakan mikropipet
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