Histogram analysis parameters of dynamic contrast-enhanced magnetic resonance imaging can predict histopathological findings including proliferation potential, cellularity, and nucleic areas in head and neck squamous cell carcinoma.

Alexey Surov,Karsten Winter,Cindy Richter,Hans Jonas Meyer,Anne-Kathrin Höhn,Leonard Leifels

doi:10.18632/oncotarget.24920

Alexey Surov, Karsten Winter + Show 4 more

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https://doi.org/10.18632/oncotarget.24920

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Journal: Oncotarget	Publication Date: Apr 20, 2018
Citations: 16	License type: cc-by

Affiliation: University Hospital Leipzig

Abstract

Our purpose was to analyze possible associations between histogram analysis parameters of dynamic contrast-enhanced magnetic resonance imaging DCE MRI and histopathological findings like proliferation index, cell count and nucleic areas in head and neck squamous cell carcinoma (HNSCC).30 patients (mean age 57.0 years) with primary HNSCC were included in the study. In every case, histogram analysis parameters of Ktrans, Ve, and Kep were estimated using a mathlab based software. Tumor proliferation index, cell count, and nucleic areas were estimated on Ki 67 antigen stained specimens. Spearman's non-parametric rank sum correlation coefficients were calculated between DCE and different histopathological parameters.KI 67 correlated with Ktrans min (p = −0.386, P = 0.043) and s Ktrans skewness (p = 0.382, P = 0.045), Ve min (p = −0.473, P = 0.011), Ve entropy (p = 0.424, P = 0.025), and Kep entropy (p = 0.464, P = 0.013). Cell count correlated with Ktrans kurtosis (p = 0.40, P = 0.034), Ve entropy (p = 0.475, P = 0.011). Total nucleic area correlated with Ve max (p = 0.386, P = 0.042) and Ve entropy (p = 0.411, P = 0.030).In G1/2 tumors, only Ktrans entropy correlated well with total (P =0.78, P =0.013) and average nucleic areas (p = 0.655, P = 0.006). In G3 tumors, KI 67 correlated with Ve min (p = −0.552, P = 0.022) and Ve entropy (p = 0.524, P = 0.031). Ve max correlated with total nucleic area (p = 0.483, P = 0.049). Kep max correlated with total area (p = −0.51, P = 0.037), and Kep entropy with KI 67 (p = 0.567, P = 0.018).We concluded that histogram-based parameters skewness, kurtosis and entropy of Ktrans, Ve, and Kep can be used as markers for proliferation activity, cellularity and nucleic content in HNSCC. Tumor grading influences significantly associations between perfusion and histopathological parameters.

Highlights

Dynamic contrast-enhanced magnetic resonance imaging (DCE MRI) is a modality to characterize perfusion and vascularization of tissues [1,2,3]
Ktrans kurtosis correlated with cell count (P = 0.40, P = 0.034)
Ve min correlated with KI 67 (P = -0.473, P = 0.011) and Ve max with total nucleic area (P = 0.386, P = 0.042)

Summary

Introduction

Dynamic contrast-enhanced magnetic resonance imaging (DCE MRI) is a modality to characterize perfusion and vascularization of tissues [1,2,3]. Parameters of DCE MRI can differentiate between malignant and benign lesions in several organs [1, 3]. DCE MRI can distinguish low and high grade tumors [4,5,6]. Some reports identified significant associations between DCE MRI findings and histopathology in several malignancies [7,8,9]. Jain et al found that DCE MRI was associated with proliferation index KI 67 in glioma [8]. In HNSCC, it has been shown that DCE MRI parameters reflected well microvessel density [9]

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