Abstract

The foliar cavities of the water fern Azolla filiculoides have as many as 20 – 25 simple hairs (SH) protruding from the epidermal cells that delimit the cavity. These SH have a transfer-like ultrastructure normally associated with secretion of metabolites. The aim of this study was the chemical characterization of the compounds that accumulated in the SH of the zones F1-12 (from 1st to 12th leaves) and F13 (from 13th until the end of the sporophyte) throughout the seasons during a 1-year study. The histochemical tests show that the vacuoles of simple hairs contain a mixture of lipids, unsaturated lipids, polysaccharides, polyphenols (o-dihydroxyphenols, phenols with free ‒OH groups and tannins) and alkaloids or alkaloid-like compounds. These substances do not show seasonal variation, having been present throughout the one-year study. The histochemical analysis demonstrated that the SH always have a variety of metabolites. The function within the Azolla-Anabaena symbiosis is not known.

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